FIG. 4.

ELISA of the senX3-regX3 IR PCR products. Biotinylated oligonucleotides O77 and O53R were immobilized on streptavidin-coated microtiter plates and incubated with 10 μl of the DIG-labeled senX3-regX3 IR PCR products obtained from M. tuberculosis strains 1033 (wells 1), 1035 (wells 2), 1036 (wells 3), 1037 (wells 4), 1038 (wells 5), 1039 (wells 6), and 2296207 (wells 7) and the Japanese (wells 9), 1173P2 (wells 10), Glaxo (wells 11), Russian (wells 12), Danish (wells 13), Prague (wells 14) and Montreal (wells 15) BCG strains. Wells 8 contained no PCR product, and wells 16 contained neither the PCR product nor the oligonucleotides.