Figure 4.

A1207 tumor regression is associated with scant granulocytes and increased activated CD8 T cells. (A) A1206 or A1207 cells were transplanted into C57BL/6 mice and size monitored for 13 days. On day 13, mice were sacrificed and tumors weighed. ****p<0.0001, determined by unpaired Student’s t-test. (B–C) Twelve A1206 and 12 A1207 tumors were dissociated 13 days after implantation, the cells pooled, processed and stained for CyTOF analysis (using antibody panel 2) as described in methods. (B) tSNE analysis was performed to identify immune cell clusters in A1206 and A1207 tumors. (C) The live, CD45⁺, CD8⁺ population were further analyzed for levels of Tnfα, Ifnγ and Gzmb to determine CD8⁺ T-cell activation. t-SNE, t-distributed stochastic neighbor embedding.