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. 2023 Aug 29;6(5):77. doi: 10.3390/mps6050077

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Diagram of the steps included in the protocol to improve the diversification and/or the production yield of fungal secondary metabolites. Step 1: recovery of the fungal culture; step 2: performing cultures of the selected fungal strain under different variables (time of growth, temperature of growth, culture nutrients concentration, culture salts concentration, and mode of growth (shaking or still)); step 3: extraction and SPE clean-up of extracts from the fungal cultures; step 4: analysis of SPE fractions by UHPLC-HRMS/MS or by HPLC-UV-MS; step 5: chemometrics analysis; step 6: selection of the improved conditions of fungal cultures growth for a large scale culture towards the isolation of secondary metabolites. This figure was prepared using a free version of BioRender^®.