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. 2021 Aug 28;1:9. doi: 10.1186/s43897-021-00011-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Validation of SlDof1 direct target genes by ChIP-qPCR. (A) The raw chromatin immunoprecipitation sequencing peaks from three biological replicates for indicated genes are shown. Black dot-line rectangles mark the peak regions that were detected within 2 kb upstream of the transcription start site in all three biological replicates. The structure of the corresponding gene is presented below with black bars representing exons and lines representing introns. The direction of transcription is indicated by arrows. (B) ChIP-qPCR analysis reveals that SlDof1 binds to the promoter regions of selected genes. The promoter structures of the target genes are shown. Blue boxes represent SlDof1 binding motifs. Green lines indicate the region used for ChIP-qPCR. All primers were designed based on the peak sequences enriched in ChIP sequencing. Values are the percentage of DNA fragments that co-immunoprecipitated with anti-SlDof1 antibodies or non-specific antibodies (preimmune IgG) relative to the input DNAs. Error bars represent the SD of three independent experiments. Asterisks indicate significant differences (P < 0.05; Student’s t-test). ACS2, ACC synthase 2; NR, never-ripe; PG2A, polygalacturonase A; NOR, nonripening; MC, macrocalyx; SlREV8, a homolog of Arabidopsis REVEILLE8; SlDIV2, a homolog of Arabidopsis DIVARICATA; SlIAA2, Aux/IAA 2; SlIAA4, Aux/IAA 4; SlIAA27, Aux/IAA 27