Fig. 2.

Schematic diagram represents general plant proteomics workflows. Proteins extracted from plant materials are fractionated using either gel-based or gel-free techniques. Three major types of proteomics analysis for identification and quantitation of posttranslational modifications, protein-protein interaction and quantitative proteomics were presented. The MS raw data generated from each workflow using different mass spectrometric techniques are carried out by database search, statistical analysis and bioinformatics analysis. Finally, the outcomes are to be further validated for either generating biological hypothesis or validation of initial mechanistic hypothesis