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. 2023 Sep 8;11:1234340. doi: 10.3389/fbioe.2023.1234340

FIGURE 2.

FIGURE 2

3D printing design and fabrication of PCL auricle structure (Aa), preparation of cell-laden alginate solution and coating process (Ab), crosslinking and rising processes (Ac), and incubation for up to 28 days (Ad). In vitro experimental groups such as ASC 2D culture in alginate (A1), ASC/chondrocyte 2D co-culture in alginate (A2), ASC 3D culture in alginate loaded on PCL structure (A3) and ASC/chondrocyte 3D co-culture in alginate loaded on PCL structure (A4). Calcium ion crosslinking of alginate and optical images showing viscose property before and after the crosslinking process (Ba), rheological property before and after the cross-linking process (Bb), optical and SEM images of cell-laden auricle scaffold (Bc) and strut/pore size analysis of the PCL auricle scaffold (Bd). Reproduce with permission from (Jang et al., 2020) under right Links from ScienceDirect.