Fig. 4.

Assessment of BOA activity against emerging variants of concern. (A) World-wide prevalence of ancestral SARS-CoV-2 (gray) and Alpha (indigo), Beta (purple), Gamma (blue), Delta (teal), and Omicron BA.1 (orange) variants of concern in 2021 [Data from Nextstrain database (40)]. (B) Sequence conservation and diversity across variants at positions that possess glycosylated Asn residues. The boxes are colored by glycotype, >90% oligomannose occupancy (lime), 50 to 75% oligomannose occupancy (olive), and complex (violet), with positions shown by numbers in the bottom right corner. In addition to Asn residues, amino acids at positions 484 in the RBD and 681 in the furin cleavage site are shown in gray boxes, with amino acids that are changed in variants of concern labeled in one letter code. (C and D) Infectivity data for several variants of concern using both authentic SARS-CoV-2 (C; Delta and Omicron) and pseudotyped viruses (D; Alpha, Beta, Gamma, Delta, and Omicron).