The 16S rRNA PCR products were spotted, in duplicate, onto two identical membranes which were hybridized with either A. felis- or B. henselae-specific probes. 1, B. henselae DNA; 2 to 4, representative samples from CSD patients; 5, pus from a non-CSD patient; 6, A. felis AfTA-1 DNA; 7, water.