TABLE 1.

Base sequences, gene locations, and predicted sizes of PCR products for the S. aureus exotoxin-specific oligonucleotide primers

Genea	Primer	Oligonucleotide sequence (5′→3′)	Nucleotide location within gene	Size of PCR product (bp)
sea	SEA-3b	CCT TTG GAA ACG GTT AAA ACG	487–507	127
	SEA-4b	TCT GAA CCT TCC CAT CAA AAA C	592–613
seb	SEB-1c	TCG CAT CAA ACT GAC AAA CG	634–653	477
	SEB-4b	GCA GGT ACT CTA TAA GTG CCT GC	1088–1110
secd	SEC-3b	CTC AAG AAC TAG ACA TAA AAG CTA GG	665–690	271
	SEC-4b	TCA AAA TCG GAT TAA CAT TAT CC	913–935
sed	SED-3b	CTA GTT TGG TAA TAT CTC CTT TAA ACG	354–380	319
	SED-4b	TTA ATG CTA TAT CTT ATA GGG TAA ACA TC	644–672
see	SEE-3b	CAG TAC CTA TAG ATA AAG TTA AAA CAA GC	482–510	178
	SEE-2c	TAA CTT ACC GTG GAC CCT TC	640–659
tst	TST-3	AAG CCC TTT GTT GCT TGC G	51–69	445
	TST-6	ATC GAA CTT TGG CCC ATA CTT T	474–495
eta	ETA-3b	CTA GTG CAT TTG TTA TTC AAG ACG	374–397	119
	ETA-4b	TGC ATT GAC ACC ATA GTA CTT ATT C	468–492
etb	ETB-3b	ACG GCT ATA TAC ATT CAA TTC AAT G	51–75	262
	ETB-4	AAA GTT ATT CAT TTA ATG CAC TGT CTC	286–312

^aSequences and locations were derived from the published nucleotide sequences for SEA (7), SEB (18), SEC₁ to SEC₃ (10, 13), SED (2), SEE (14), TSST-1 (8), and ETA and ETB (20). 

^bModified oligonucleotide primers based on primer sequences published by Johnson et al. (17). 

^cOligonucleotide primer sequences as described by Johnson et al. (17). 

^dThe primer sequences used here cover all three subtypes of SEC.