TABLE 3.
Results of testing reference strains for staphylococcal enterotoxin genotype derived from agarose gel analysis of multiplex PCR and colorimetric microtiter plate DNA EIA
Reference strain | Toxin phenotype | Result by:
|
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Agarose gel analysisa
|
Colorimetric assay (OD450/630 ratio)
|
||||||||||
sea | seb | sec | sed | see | sea | seb | sec | sed | see | ||
KN543 | SEA | + | − | − | − | − | 0.853 | 0.037 | 0.062 | 0.021 | 0.018 |
KN556 | SEA | + | − | − | − | − | 0.799 | 0.036 | 0.062 | 0.019 | 0.019 |
KN560a | SEA | + | − | − | − | − | 1.035 | 0.063 | 0.030 | 0.030 | 0.018 |
H390 | SEA, SED | + | − | − | + | − | 0.867 | 0.041 | 0.063 | 0.817 | 0.019 |
ATCC 14458 | SEB | − | + | − | − | − | 0.021 | 1.364 | 0.117 | 0.050 | 0.022 |
62/92b | SEB | − | + | − | − | − | 0.018 | 1.169 | 0.066 | 0.054 | 0.036 |
H288a | SEB | − | + | − | − | − | 0.028 | 1.540 | 0.037 | 0.140 | 0.018 |
st 20783 | SEB | − | + | − | − | − | 0.016 | 0.981 | 0.055 | 0.041 | 0.021 |
ATCC 19095 | SEC | − | − | + | − | − | 0.015 | 0.043 | 1.335 | 0.021 | 0.025 |
1229/93b | SEC | − | − | + | − | − | 0.015 | 0.038 | 1.406 | 0.022 | 0.020 |
H494 | SEC | − | − | + | − | − | 0.021 | 0.024 | 2.200 | 0.026 | 0.018 |
ATCC 23235 | SED | − | − | − | + | − | 0.013 | 0.042 | 0.123 | 1.214 | 0.021 |
1634/93b | SED | − | − | − | + | − | 0.019 | 0.024 | 0.128 | 1.475 | 0.018 |
ATCC 27664 | SEE | − | − | − | − | + | 0.021 | 0.034 | 0.099 | 0.020 | 1.819 |
Cowan 1c | − | − | − | − | − | 0.014 | 0.083 | 0.039 | 0.019 | 0.017 | |
ATCC 20044d | − | − | − | − | − | 0.020 | 0.031 | 0.110 | 0.024 | 0.018 |
−, negative; +, positive (judged by eye).
Kindly provided by W. Witte (Robert Koch-Institut Wernigerode, Germany).
S. aureus negative control strain.
S. epidermidis negative control strain.