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. 1998 Sep;36(9):2548–2553. doi: 10.1128/jcm.36.9.2548-2553.1998

TABLE 3.

Results of testing reference strains for staphylococcal enterotoxin genotype derived from agarose gel analysis of multiplex PCR and colorimetric microtiter plate DNA EIA

Reference strain Toxin phenotype Result by:
Agarose gel analysisa
Colorimetric assay (OD450/630 ratio)
sea seb sec sed see sea seb sec sed see
KN543 SEA + 0.853 0.037 0.062 0.021 0.018
KN556 SEA + 0.799 0.036 0.062 0.019 0.019
KN560a SEA + 1.035 0.063 0.030 0.030 0.018
H390 SEA, SED + + 0.867 0.041 0.063 0.817 0.019
ATCC 14458 SEB + 0.021 1.364 0.117 0.050 0.022
62/92b SEB + 0.018 1.169 0.066 0.054 0.036
H288a SEB + 0.028 1.540 0.037 0.140 0.018
st 20783 SEB + 0.016 0.981 0.055 0.041 0.021
ATCC 19095 SEC + 0.015 0.043 1.335 0.021 0.025
1229/93b SEC + 0.015 0.038 1.406 0.022 0.020
H494 SEC + 0.021 0.024 2.200 0.026 0.018
ATCC 23235 SED + 0.013 0.042 0.123 1.214 0.021
1634/93b SED + 0.019 0.024 0.128 1.475 0.018
ATCC 27664 SEE + 0.021 0.034 0.099 0.020 1.819
Cowan 1c 0.014 0.083 0.039 0.019 0.017
ATCC 20044d 0.020 0.031 0.110 0.024 0.018
a

−, negative; +, positive (judged by eye). 

b

Kindly provided by W. Witte (Robert Koch-Institut Wernigerode, Germany). 

c

S. aureus negative control strain. 

d

S. epidermidis negative control strain.