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[Preprint]. 2023 Sep 16:2023.09.16.558055. [Version 1] doi: 10.1101/2023.09.16.558055

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Extended data Fig 1: — TcdA is actively secreted in high toxin producer strains. a. extracellular/intracellular ratio of TcdA from 630Δerm, UK1 and VPI10463 strains. The strains were grown in TY medium and TcdA concentrations from the supernatant and the intracellular content were quantified using a TcdA-ELISA. Means and SD are shown; n=3 independent experiments. **P ≤ 0.01, ***P ≤ 0.001 and *** P ≤ 0.0001 by a one-way ANOVA. b. TcdA titers in extracellular and intracellular fractions of E25 and CD21–013 strains after 12 and 24 hours of growth. The strains were grown in TY medium and TcdA was quantified using TcdA-ELISA. Means and SD are shown; n=3 independent experiments. ***P ≤ 0.001 and *** P ≤ 0.0001 by a one-way ANOVA. c. extracellular/intracellular ratio of TcdA from E25 and CD21–013 strains. The strains were grown in TY medium and TcdA concentrations from the supernatant and the intracellular content were quantified using a TcdA-ELISA. Means and SD are shown; n=3 independent experiments. ***P ≤ 0.001 by a one-way ANOVA.