Extended data Table 1.
Strains and plasmids used in this study.
| Strain | Genotype | Origin |
|---|---|---|
|
| ||
| E. coli | ||
|
| ||
| NEB-10 beta | Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 | New England Biolabs |
| e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC) | ||
| HB101 (RP4) | supE44 aa14 galK2 lacY1 Δ(gpt-proA) 62 rpsL20 (StrR)xyl-5 mtl-1 recA13 Δ(mcrC-mrr) hsdSB (rB-mB-) RP4 (Tra+ IncP ApR KmR TcR) | Laboratory stock |
| EC1370 | HB101(RP4) carrying pDIA7080 | This work |
| EC1384 | HB101(RP4) carrying pDIA7090 | This work |
| C. difficile | ||
| 630Δerm | 630 ΔermB | Laboratory stock1 |
| UK1 | Laboratory stock2 | |
| VPI10463 | Laboratory stock3 | |
| E25 | Laboratory stock4 | |
| CD21–013 | Laboratory stock5 | |
| CDIP1688 | VPI10463 ΔtcdE | This work |
| CDIP1855 | VPI10463 carrying pMC358 | This work |
| CDIP1920 | VPI10463 ΔtcdE carrying pMC358 | This work |
| CDIP1753 | UK1 ΔtcdE | This work |
| CDIP1838 | UK1 carrying pMC358 | This work |
| CDIP1854 | UK1 ΔtcdE carrying pMC358 | This work |
| CNRS_CD166 | VPI10463 carrying p112 | This work |
| CNRS_CD170 | VPI10463 ΔtcdE carrying p112 | This work |
| CNRS_CD167 | VPI10463 carrying p113 | This work |
| CNRS_CD171 | VPI10463 ΔtcdE carrying p113 | This work |
| CNRS_CD169 | VPI10463 carrying p115 | This work |
| CNRS_CD172 | VPI10463 ΔtcdE carrying p115 | This work |
| CDIP1834 | UK1 carrying p112 | This work |
| CDIP1850 | UK1 ΔtcdE carrying p112 | This work |
| CDIP1837 | UK1 carrying p113 | This work |
| CDIP1853 | UK1 ΔtcdE carrying p113 | This work |
| CDIP1836 | UK1 carrying p115 | This work |
| CDIP1852 | UK1 ΔtcdE carrying p115 | This work |
|
| ||
| Plasmid | ||
| pMSR0 | Allele exchange in C. difficile UK1 and VPI10463 | Peltier et al6 |
| pMC358 | Promoterless phoZ | Edwards et al7 |
| pDIA7080 | pMSR0 derivative for tcdE deletion in VPI10463 | This work |
| pDIA7090 | pMSR0 derivative for tcdE deletion in UK1 | This work |
| p112 | pMC358 derivative for PtcdR-phoZ fusion | This work |
| p113 | pMC358 derivative for PtcdB-phoZ fusion | This work |
| p115 | pMC358 derivative for PtcdA-phoZ fusion | This work |
1.
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2.
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Anjou C et al., in preparation
6.
Peltier, J. et al. (2020) Commun Biol 3, 718.
7.
Edwards, A.N. et al. (2015). Anaerobe 32, 98–104.