Extended Data Fig. 8 |. Effect of transcription level, gene length, and burst duration in transcription burst kinetics.

a, Distribution of distances between consecutive RNA polymerases in the first 10 kb of the gene body in single cells compared with distances from permuted data (randomized cell ID but unchanged read position, left) or uniform data (randomized read position along the gene but unchanged cell ID, right). Distances up to 2.5 kb are shown. b, Test of our burst kinetics model by simulating burst size and burst frequency. c, Correlation between the burst frequency from scGO-seq (left) and intron seqFISH (right) with the burst frequency from scRNA-seq. d, Test of our burst kinetics model by simulation using inferred burst size and burst frequency. e, Correlation between the burst size greater than one and the burst frequency of genes. f, The effect of gene length (from 100 bp to 10 kb after trimming 500 bp on either end of the genes) on burst size and frequency. g, Correlation between burst frequencies calculated from the burst window of either the first 5 kb or the first 10 kb gene bodies.