| Steps | Denature (98°C) | Anneal (60°C) | Extension (72°C) | Storage (4°C) | |
|---|---|---|---|---|---|
| Initial denaturation | 30 sec | - | - | - | |
| 6 cycles | Denaturation | 10 sec | - | - | - |
| Anneal | 20 sec | - | - | ||
| Extension | 30 sec | ||||
| Final extension | - | - | 2 min | - | |
| Storage | - | - | - | ∞ |
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