Table 1.
Antigen detection with infectious virus.
| α-A29a | S31 a | S27 a | ||||||
|---|---|---|---|---|---|---|---|---|
| 4B4 b | S27 b | S27 b | S31 b | |||||
| 2°-biotin | 2°-PE | 2°-biotin | 2°-PE | 2°-biotin | 2°-PE | 2°-biotin | 2°-PE | |
| MPVX Zaire ′79 | 1706.6 | 405.4 | 701.5 | 845.4 | 1.1 | 0.9 | 1.0 | 0.0 |
| MPXV Katakombe | 1916.7 | 436.2 | 792.8 | 920.0 | 1.2 | 0.9 | 1.0 | 0.0 |
| MPXV US 2003 | 1.1 | 219.3 | 829.5 | 807.0 | 2.7 | 1.5 | 1.0 | 0.0 |
| MPXV current | 1.1 | 458.0 | 904.1 | 774.6 | 6.2 | 1.1 | 1.0 | 0.0 |
| VACV lister | 39.1 | 33.7 | 38.3 | 23.6 | 1.3 | 0.4 | 1.0 | 0.0 |
| VACV IHDJ | 17.5 | 14.2 | 14.9 | 10.6 | 1.2 | 0.9 | 1.0 | 0.0 |
| VACV WR | 32.9 | 27.1 | 49.9 | 26.7 | 1.5 | 0.6 | 1.0 | 0.0 |
| Cowpox BR | 46.8 | 37.1 | 53.4 | 34.6 | 1.5 | 0.6 | 1.0 | 0.0 |
| Cowpox wt | 11.4 | 11.7 | 13.2 | 10.4 | 1.1 | 0.9 | 1.0 | 0.0 |
| Camelpox Somalia | 1.0 | 0.8 | 1.2 | 1.1 | 1.0 | 0.9 | 1.0 | 0.1 |
aThe 1° MAb is covalently linked to magnetic microspheres.
bThe 2° MAb is biotinylated (detection with SAPE) or directly PE-labeled.