Skip to main content
. Author manuscript; available in PMC: 2023 Dec 16.
Published in final edited form as: ACS Synth Biol. 2022 Nov 15;11(12):4103–4112. doi: 10.1021/acssynbio.2c00405

Figure 3: CRISPRi with PAM-flexible dCas9 variants.

Figure 3:

(A) CRISPRi for PAM-flexible dCas9 variants was tested on an mRFP reporter gene with sgRNAs targeting the promoter (119) or coding sequence (RR1 and RR2), where each target site encodes an NGG PAM. (B) Fold-repression of the mRFP reporter gene with a single expressed sgRNA. (C & D) Comparison of fold-repression with one or two sgRNAs expressed. Fold-repression consistently increases when two sgRNAs are expressed. See Figure S6 for a comparison of all dCas9 variants, including dCas9-NG and dSpG, with single and multiple sgRNAs. Values in panel B, C, and D represent the mean ± standard deviation calculated from n = 3.