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. Author manuscript; available in PMC: 2024 May 1.
Published in final edited form as: Adv Ther (Weinh). 2023 Feb 16;6(5):2200219. doi: 10.1002/adtp.202200219

Figure 2. Identification of nanoparticles effective for in vitro delivery of SAM to C2C12 murine myoblasts.

Figure 2.

(A) A library of 196 PBAEs were synthesized combinatorially from 28 base acrylate terminated polymers and 7 end-cap monomers. Polymers were screened in 384-well plates in C2C12 cells for transfection at a dose of 1 ng of eGFP SAM per well at two w/w ratios. Each cell of the heatmap shows mean of two wells of a 384-well plate. Transfection of myoblasts using eGFP SAM was strongly improved compared to 5mou eGFP mRNA used at the same dose as visible by (B) Microscopy (25 ng per well) and (C) Quantified percent transfection and (D) Selected nanoparticles for follow-up dose-titration screening were potent down to 20.6 pg/well in a 96-well format.