FIG. 1.

Diagram illustrating the AFLP technique used in the study. (A) The double-stranded fragment produced from digestion with HindIII (recognition site A↓AGCTT). (B) Sequences of the two complementary oligonucleotides forming the adapter which is ligated to each end of the HindIII restriction fragment. Nucleotides which have been inserted into the adapter sequence to eliminate the restriction site after ligation are underlined. (C) Fragment formed following ligation to the adapter. The double-headed arrow marks the point where ligation occurs. (D) Fragments present after initial denaturation at 94°C. (E) Initial amplification product. Newly synthesized DNA is shaded. (F) Primers used in subsequent amplification reactions. These are based on the adapter sequence. Selectivity is conferred by the final 3′ base of each primer.