FIGURE 3.

Microbial alpha and beta diversity was observed across different experimental groups (n = 6). (A) Microbial diversity among different treatment groups across four time points, (B) overall diversity across different treatment independent of time (C) taxa richness among different treatment groups across four time points, (D) overall richness across different treatment independent of time. The symbol “#” represents p < 0.05 vs. control group and the symbol “*” represents p < 0.05 vs. western diet (WD) group according to Kruskal–Wallis test; ns represents non-significant. All the alpha diversity measures along with the metadata information are provided in the Supplementary Table 2. (E,F) PCoA analysis was performed on Bray–Curtis distances calculated on relative abundance of each species. (E) All experimental groups at different time points were considered. This shows the strong differences due to diet (PERMANOVA, r² = 0.29) as compared to the timepoints (PERMANOVA, r² = 0.21) and (F) differences between experimental groups at each time point such as week 4 (top left), week 8 (top right), week 12 (bottom left), and week 16 (bottom right) were observed. Strong differences (PERMANOVA, r² = 0.56–0.67) were observed among the treatment groups at each time point.