Figure 1.

HDAC6 is involved in DSB repair. (A) pEJ5-GFP U2OS or DR-U2OS cells were transfected with HDAC6 or negative control (NC) siRNAs and subjected to NHEJ and HR assays, separately. (B) pEJ5-GFP U2OS or DR-U2OS cells were transfected with Myc-HDAC6 or empty vector and subjected to NHEJ and HR assays, separately. (C, D) HeLa cells were transfected with HDAC6 or NC siRNAs for 48 h, treated with 10 μM VP16 for 2 h, and washed three times with fresh medium before recovery for the indicated times. All samples were then subjected to a comet assay. Representative images (C) and statistical analyses (D) are shown. (E, F) HeLa cells were transfected with Flag-HDAC6 plasmids for 48 h. After 3 Gy irradiation, the cells were counted and seeded for the colony formation assays. For the control (Ctr) group, 500 cells were seeded in each plate. For the IR-treated group, 5000 cells were seeded in each plate. Representative images (E) and statistical analyses (F) are shown. (G, H) HDAC6 WT or HDAC6 KO HCT116 cells underwent 3 Gy irradiation (IR) and were counted and seeded for colony formation assays. For the control (Ctr) group, 500 cells were seeded in each plate. For the IR-treated group, 5000 cells were seeded in each plate. Representative images (G) and statistical analyses (H) are shown. All data represent the means ± SD (n = 3, *P< 0.05, **P< 0.01, ***P< 0.001).