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. 2023 Jul 28;51(17):9166–9182. doi: 10.1093/nar/gkad631

Figure 3.

Figure 3.

HDAC6 is associated with the H2A/H2A.X ubiquitination signaling cascade in DSB repair. (A–E) All cells were irradiated with 10 Gy followed by release for 1 h, after transfection with the indicated plasmids or siRNAs. (A) HeLa cells were transfected with Myc-HDAC6 or an empty plasmid for 48 h. Whole cell lysate and chromatin fractions were subjected to western blotting. (B) HeLa cells were transfected with HDAC6 or negative control (NC) siRNAs for 48 h. Whole cell lysate and chromatin fractions were subjected to western blotting. (C) HDAC6 KO HCT116 cells were transfected with Myc-HDAC6 or empty plasmid for 48 h. Whole cell lysate and chromatin fractions were subjected to immunoblotting. (D, E) HeLa cells were transfected with HDAC6 or negative control (NC) siRNAs for 48 h. The cells were then fixed, and the samples were labeled with the indicated antibodies. Representative images (D) and statistical analyses (E) are shown. The data represent the means ± SD (n = 5, *P< 0.05, **P< 0.01, ***P< 0.001).