Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 10;51(17):9227–9247. doi: 10.1093/nar/gkad648

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Increased telomeric damage in G7 Pot1b^−/− cells promotes telomerase recruitment to telomeres. (A) Representative images of telomerase recruitment assay in G7 Pot1b^−/− cells treated with/without 150nM AZ20, and with/without expressing vector/POT1b/POT1ba. γ-H2AX (blue) were detected by immunostaining with anti-γH2AX antibodies. hTR RNA was detected by hybridization with Cy5-hTR cDNA probes (green) and telomeres visualized by hybridization with PNA probe Cy3-OO-(CCCTAA)₃ (red). Scale bar: 5μm. (B) Quantification of telomerase and telomere colocalization in (A). Data show the mean ± 95% CI of the mean from at least two experiments with 150 nuclei analyzed per cell line. p-values are shown and generated from one-way ANOVA analysis followed by Tukey's multiple comparison. (C) Quantification of the indicated conditions with ≥ 5 γ-H2AX TIFs in (A). Data show the mean ± standard deviation from at least two independent experiments in which 150 nuclei were analyzed per cell line. P-values are shown and generated from one-way ANOVA analysis followed by Tukey's multiple comparison. (D) Representative images of telomerase recruitment assay in G3 Pot1b^−/− cells treated with/without 150nM AZ20, with/without expressing POT1ba. γ-H2AX were detected by immunostaining with anti-γH2AX antibodies (blue). hTR RNA was detected by hybridization with Cy5-hTR cDNA probes (green) and telomeres visualized by hybridization with PNA probe Cy3-OO-(CCCTAA)₃ (red). Scale bar: 5 μm. (E) Quantification of telomerase and telomere colocalization in (D). Data show the mean ± 95% CI of the mean with 150 nuclei analyzed per cell line. P-values are shown and generated from one-way ANOVA analysis followed by Tukey's multiple comparison. (F) Quantification of the indicated conditions with ≥5 γ-H2AX TIFs in (D). Data show the mean from 150 nuclei analyzed per cell line.