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. 2023 Aug 10;51(17):9227–9247. doi: 10.1093/nar/gkad648

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Telomerase activity is required to promote telomeric damage in late-generation Pot1b^−/− cells. (A) Representative images of γ-H2AX TIFs in the indicated cell lines. Cells were immunostained with γ-H2AX antibody (green), TRF2 antibody (red) and DAPI to stain nuclei (blue). Scale bar: 5μm. (B) Quantification of the indicated cell lines with ≥5 γ-H2AX TIFs in (A). Data show the mean ± standard deviation from two independent experiments in which 150 nuclei were analyzed per cell line. P-values are shown and generated from one-way ANOVA analysis followed by Tukey's multiple comparison. (C) Representative images of γ-H2AX TIFs in the indicated cell lines expressing the indicated constructs. Cells were immunostained with γ-H2AX antibody (green), TRF2 antibody (red) and DAPI to stain nuclei (blue). Scale bar: 5μm. (D) Quantification of the indicated cell lines with ≥ 5 γ-H2AX TIFs in (C). Data show the mean ± standard deviation from three independent experiments in which 150 nuclei were analyzed per cell line. P-values are shown and generated from one-way ANOVA analysis followed by Tukey's multiple comparison. (E) Schematic of mTERT showing the K78 and K560 amino acids.