Skip to main content
. 2023 Aug 18;42(39):2878–2891. doi: 10.1038/s41388-023-02809-0

Fig. 3. FBXO28 negatively regulates SNAI2 protein levels and function.

Fig. 3

A Huh7 cells transfected with FBXO28-Flag or vector for 48 h (left panel) or Hep3b infected with lentivirus expressing shFBXO28 or shCtrl for 72 h (right panel) were subjected to IB analyses of the EMT pathway-related markers. B Total cellular RNA was extracted for RT-qPCR analysis in the same conditions as in (A). Data were presented as mean ± SD from three independent experiments, *P < 0.05 and **P < 0.01 (independent T-test). C Huh7 cells expressing FBXO28-Flag or vector for 72 h by transient transfection were treated with MG132 for 6 h prior to IB analyses. D, E Huh7 cells transfected with FBXO28-Flag or a control vector (D) or Hep3b cells expressing shFBXO28 or shCtrl for 48 h (E) were treated with CHX (10 μg/mL) for the indicated time interval and subjected to IB analyses. The SNAI2 protein levels in (D and E) were quantified, respectively, and shown on the right panel. F Huh7 cells stably expressing FBXO28-mGFP or a control vector were transfected with SNAI2-HA for 48 h; or were transfected with SNAI2-HA or a control vector for 24 h prior to transfection with FBXO28-Flag for 24 h, followed by migration and invasion assay, respectively. Representative images are shown. Scale bar: 10 μm. Data were expressed as mean ± SD from three independent experiments. *P < 0.05 and **P < 0.01 (one-way ANOVA).