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. 2023 Sep 22;14:5901. doi: 10.1038/s41467-023-41654-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a GloSensor cAMP accumulation assay and NanoBiT mini-Gs recruitment assay performed in Expi293F cells transiently expressing any of 81 oGPCRs or D1R with relative luminescence unit (RLU) value shown. Receptors that show high cAMP levels compared with the other oGPCRs are colored in red. b Sequence alignment of ICL2 in caoGPCRs, D1R and β2AR. Residues are highlighted based on the Clustal color scheme. c Concentration–response curves in the cAMP accumulation assay at Expi293F cells transiently transfected with GPR174 or D1R treated with lysoPS or dopamine. Each data point represents mean ± SEM from three independent experiments. Source data are provided as a Source Data file.