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. 2023 Sep 22;14:5901. doi: 10.1038/s41467-023-41654-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Cryo-EM map of the GPR174 and mini-G_s complex. b Ribbon representation of the GPR174 and mini-G_s complex. LysoPS and cholesterol are shown as sticks. c Cryo-EM density map of lysoPS and cholesterol in the GPR174‒G_s complex at a contour level of 3.5σ. d Lipidomics analysis of the GPR174‒G_s and D1R‒G_s complexes by MS. The relative abundance of representative LPs with the acyl chain length from 16:0 to 20:1 determined by MS is shown. Source data are provided as a Source Data file.