FIG. 1.

Sensitivity of the PCR for detection of EHDV with primers from NS3 genome sequence of EHDV-1. (A) Visualization of the 535-bp EHDV-specific PCR product on an ethidium bromide-stained agarose gel from 1.0 pg of EHDV RNA. Lanes: MW, molecular weight marker; 1 to 5, 10 pg, 1.0 pg, 100 fg, 10 fg, and 1.0 fg, respectively, of EHDV-1; 6, BHK-21 total nucleic acid extract. (B) Southern blot with chemiluminescent hybridization of the gel shown in panel A showing detection of as little as 10 fg of EHDV-1 RNA.