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Specificity of the PCR for RNA from the EHDV NS3 genome. Amplification product was not detected from a high concentration of 1.0 ng of BTV RNA from North American BTV prototype viruses or total nucleic acid extracts from BHK-21 cells. Lanes: MW, molecular weight marker; 1, 1.0 pg of EHDV-1; 2 to 6, BTV-2, -10, -11, -13, and -17, respectively; 7, BHK-21 total nucleic acid extract.
