FIG. 4.

Detection of the 535-bp EHDV NS3-specific PCR product by Southern blotting with chemiluminescent hybridization from clinical samples. Lanes: MW, molecular weight marker; 1, spleen homogenate from EHDV-2-infected deer; 2, lung homogenate from EHDV-2-infected deer; 3 and 4, unfractionated lysed blood cells from a calf experimentally infected with EHDV-1; 5 and 6, unfractionated lysed blood from a calf experimentally infected with EHDV-2; 7 and 8, unfractionated lysed blood from uninfected calves; 9 and 10, unfractionated lysed blood cells from uninfected deer.