Table 3.
Change of unfolding Gibbs free energy due to the detected missense variants
| Variant | I-Mutant | MUPro | mCSM | Dynamut | ||||
|---|---|---|---|---|---|---|---|---|
| Stability | DDG | Stability | DDG | Effect | ΔΔG | Effect | ΔΔG | |
| p.Gly87Val | Decrease | − 0.52 kcal mol−1 | Increase | 0.006 kcal mol−1 | Destabilizing | − 0.496 kcal mol−1 | Stabilizing | 0.195 kcal mol−1 |
| p. His374Gln | Decrease | − 0.32 kcal mol−1 | Decrease | − 0.74 kcal mol−1 | Destabilizing | − 2.195 kcal mol−1 | Destabilizing | − 0.363 kcal mol−1 |
ΔΔG/DDG Gibbs free energy change score indicated increases in protein stability > 0 > decrease in protein stability