Figure 1.

Identification of mice soleus muscle proteins in response to changes in gravity loading. (A) Proteins with significantly decreased or increased levels (< 0.01, fold change ≤ 0.5 or ≥ 2), as compared to control, in three gravity change experiments in model mice. (B) The level of each soleus muscle protein from the hypergravity and control mice. The immunoblotting analysis procedure for protein detection is described in the Materials and Methods. Note that the membrane is cropped to remove irrelevant areas, and uncropped images are shown in Supplemental Fig. S2. (C) Serum Pvalb levels from HU and control mice as detected by immunoblot analysis (Supplemental Fig. S4). The protein levels were determined from the band intensity using Multi Gauge software (*p < 0.05).