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. 2023 Sep 22;14:5910. doi: 10.1038/s41467-023-41602-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Quantified number of peptides and proteins with standard DIA and HRMS1-DIA with 1 ng injection with 20PSD Evosep whisper method. Number above the bar indicated the mean identified peptide and protein group number. b Acquired MS1 and MS2 points-per-peak. Standard DIA (OT-DIA) is shown in the top and HRMS1-DIA (OT-HRMS1) in the bottom. Gray dashed line indicates a 3 point-per-peak cut off (only for visualization purposes). c CV distribution histograms of quantitative precision of standard and HRMS1-DIA. Respectively, MS2 and MS1-based quantification was used for CV comparison. d, e Barplots of quantified peptides and proteins in wide window HRMS1-DIA acquisition survey with 40SPD Evosep whisper method with 1 ng injection. f Heavy and light protein abundance ratio density plots. 1 ng total input material was kept constant and the amount of light and heavy peptides were varied to achieve the required ratios. HRMS1 MS1-based quantification is shown in the top, and DIA MS2-based quantification in the negative side of the plot. Gray dashed line marks the zero values where the measured and expected fold-change matches perfectly. Source data are provided as a Source Data file.