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. 2023 Jun 22;193(2):1213–1226. doi: 10.1093/plphys/kiad363

Figure 5.

Figure 5.

NnGST2 rescues anthocyanin deficiency in Arabidopsis tt19 mutants. A) Phenotypes of 7-d-old WT, tt19, and 35S::NnGST2/tt19 Arabidopsis seedlings and their corresponding mature seeds. Images were digitally extracted for comparison. Scale bar = 500 µm. B) HPLC chromatograms of anthocyanin profile in the WT, tt19, and 35S::NnGST2/tt19 seedlings. C) Anthocyanin contents in the WT, tt19, and 35S::NnGST2/tt19 seedlings calculated based on the peak area method. Double asterisks (**) indicate statistical significance at P < 0.01 (t test). Data are mean ± Sd (n = 3). The boxplot elements are defined as follows: centerline, median; box limits, upper and lower quartiles; and whiskers, 1.5× interquartile range. D) The expression of anthocyanin biosynthesis genes in the WT, tt19, and 35S::NnGST2/tt19 transgenic lines. Different alphabetical letters show significant differences at P < 0.05 (1-way ANOVA with Tukey's test). Data are mean ± Sd (n = 3).