Figure 4.
Temperature effects on heritabilities of metabolite levels. A) Broad-sense heritability, H2, as estimated for the two different temperatures and narrow-sense heritability, h2, taken as the coefficient of determination in cross-validation (average over 25 repeat runs, see Materials and Methods for details on H2 and h2 estimates). H2 values for T = 6 °C and T = 16 °C are correlated at a level of Pearson correlation coefficients r = 0.65 (P = 1.3E−5), and for h2: r = 0.39 (P = 0.017). The different heritability scales H2 and h2 are correlated at r = 0.77 (P = 2.1E−8) for T = 6 °C, and r = 0.55 (P = 4.01E−4) for T = 16 °C, respectively. B)h2 estimates based in cis (same-temperature) and trans (across different temperatures) settings, i.e. cross-validated rrBLUP estimates of coefficients of determinations, taken as h2, trained under a chosen temperature and applied either in cis (same temperatures) or trans (respective alternative temperature). Cis/trans correlations of values, T = 6 °C: r = 0.34 (P = 0.04), and for T = 16 °C: r = 0.45 (P = 0.005). Note that the absolute values of H2 and h2 should not be directly compared, as they are computed using very different procedures. Negative h2-value estimates were replaced by 0 before averaging. For graphical compactness, values have been multiplied by −1 for T = 16 °C. In both plots, metabolites were sorted according to their T = 6 °C values.