Table 2.
Passage 0 and Passage 1 Cell Growth Characteristics of Chondrocytes in the Quantum and on Tissue Culture Plastic in Human Platelet Lysate and Fetal Bovine Serum
| Cells seeded ( × 106) | Cells harvested ( × 106) | Increased cell yield ( × 106) | Days in culture | Population doublings | ||
|---|---|---|---|---|---|---|
| P0–1 | TCP-human platelet lysate | 4.1 ± 0.4 (3.5–4.4) | 13.0 ± 4.9 (5.4–18.7) | 8.9 ± 4.5 (1.9–14.3) | 13.2 ± 1.3 (11–14) | |
| TCP-fetal bovine serum | 2.0 ± 1.3 (0.4–3.5) | 6.3 ± 5.4 (0.4–13.6) | 4.4 ± 4.1 (0.03–10.1) | 16.0 ± 4.5 (11–23) | ||
| P1–2 | TCP-human platelet lysate | 1.4 ± 0.7 (0.4–2.1) | 5.9 ± 4.4 (2.2–13.2) | 4.5 ± 3.7 (1.8–11.4) | 7 ± 2.7 (3–10) | 2.1 ± 0.6 (1.5–2.9) |
| TCP-fetal bovine serum | 0.13 ± 0.93 (0.4–2.6) | 3.8 ± 3.0 (1.5–8.2) | 2.6 ± 3.2 (0.3–7.3) | 7.7 ± 4.2 | 1.8 ± 1.3 (0.1–3.2) | |
| Quantum®-human platelet lysate | 10.2 ± 3.6 (5.0–15.0) | 86.4 ± 38.5 (24.0–120.0) | 76.2 ± 34.9 (14.0–109.2) | 8.4 ± 1.5 (7–10) | 3.0 ± 1.0 (1.3–3.9) |
Data are mean ± SD, with the range displayed in brackets (n = 5).
P, passage; TCP, tissue culture plastic.