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. 2023 Sep 19;34(17-18):947–957. doi: 10.1089/hum.2023.005

Figure 1.

Figure 1.

ZF-CEM technology design and transgene induction following transfection. (A) AAV +ZF-FKBP and AAV −ZF-FKBP plasmid cassettes. (B) HEK293T cells were transfected with the plasmids depicted in A. Cells were treated with CEM87 and after 48 h, the change in luciferase luminescence was determined. At 200 nM, CEM87 was able to significantly increase luciferase transgene expression when cells were transfected with plasmids containing +ZF-FKBP. (C) Western blot of HEK293T cells transfected with +ZF-FKBP plasmid and then treated with CEM87. Western blot is detecting ZF-FKBP fusion protein with anti-FKBP primary antibody. p Value parameters: ****p ≤ 0.0001. AAV, adeno-associated virus; FKBP, FK506 binding protein; ITR, inverted terminal repeat; ZF-FKBP, ZFHD1 fused to FKBP.