Figure 7.

Tumor sections labeled for Masson trichrome staining. Different colors indicate collagen fibers (blue), cytoplasm and muscles (red), and nuclei (black). Groups included untreated tumors (control), ultrasound-stimulated microbubbles (USMB; 3%), radiation therapy (XRT, 8Gy), and combined treatments (USMB + 8Gy). The first and second row displays representative low-magnification images of trichrome staining. The scale bar represents 1 mm (B) Third and fourth row displays representative high-magnification images of trichrome staining. The scale bar represents 50 μm. Results from high magnification microscopy images revealed higher fibrotic tissues in down-regulated tumors compared to up-regulated tumor tissue. From [86].