Figure 7.

A, Experimental Timeline. Following 5 weeks of baseline drinking of 15% EtOH daily for 1h, mice underwent 4 cycles of AIR/CIE vapor with alternating weeks of daily 1h alcohol access. During drinking tests 3 and 4, mice received daily saline injections 30 min prior to drinking. On day 3 of test 4, mice were injected with 3 mg/kg CNO instead of saline. B, Viral injection schematic. C-F, Representative images of viral placements in cortical amygdala showing a subject with anterior placement (C,E) and a subject with posterior placement (D,F). G, Alcohol intake was higher in CIE than AIR mice during test weeks 3 and 4, with no effect of mCherry vs. hM4Di genotype. H, BECs were relatively stable in CIE mice across the four vapor exposure weeks. I, CNO reduced drinking selectively in CIE mice expressing hM4Di virus. J-L, Sucrose consumption (J), total fluid consumption (K), and sucrose preference (L) were unaffected by CNO (p>0.05). We detected a main effect of hM4Di virus to reduce sucrose drinking independent of CIE or CNO treatment (p<0.05). M-N, Locomotor activity measured via distance traveled (M) and mean velocity (N) did not differ based on virus or CIE exposure after CNO administration (p>0.05).