Fig. 2. Effect of the post-mortem delay (PMD) and fixation time on the detection of neural stem cells (NSCs).

a–f Representative images of SRY (sex determining region Y)-box 2 (Sox2), Vimentin, and S100 calcium-binding protein β (S100β) staining under distinct experimental conditions. g Density of Sox2⁺ S100β^- NSCs. h Density of Vimentin⁺ S100β^- NSCs. i Density of Vimentin⁺ Sox2⁺ cells. j Density of Vimentin⁺ Sox2⁺ S100β^- NSCs. In a–f, Z-projection images are shown. ML Molecular layer. GCL Granule cell layer. H Hilus. White scale bar: 10 μm. Yellow triangles: Vimentin⁺ Sox2⁺ S100β^- NSCs. Graphs represent mean values ± SEM. n = 8 mice. *0.05 > p ≥ 0.01; **0.01 > p ≥ 0.001; and ***p < 0.001.