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. 2023 Sep 23;6:978. doi: 10.1038/s42003-023-05367-z

Fig. 3. Effect of the post-mortem delay (PMD) and fixation time on the detection of markers of mature dentate granule cells (DGCs).

Fig. 3

a–f Representative images of Nissl staining showing the hippocampal anatomy under distinct experimental conditions. g–l High-power magnification images of Calbindin (CB) staining in the Granule cell layer (GCL) obtained under distinct experimental conditions. m Volume of the dentate gyrus (DG). n Total number of DGCs. o Nuclear area of DGCs. p Percentage of DGCs that express CB. In g–l, Z-projection images are shown. DG dentate gyrus. CA1-3: Cornu Ammonis 1 – 3. GCL Granule cell layer. H Hilus. Yellow scale bar: 200 μm. White scale bar: 5 μm. Magenta triangles: CB+ DGCs. Dotted line with circles: nuclear area. Graphs represent mean values ± SEM. n = 8 mice. *0.05 > p ≥ 0.01; and ***p < 0.001.