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. 2023 Sep 23;6:978. doi: 10.1038/s42003-023-05367-z

Fig. 4. Effect of the post-mortem delay (PMD) and fixation time on the detection of glial cells.

Fig. 4

a–f Representative images of Iba1 staining showing microglial cells under distinct experimental conditions. g–l Representative images of S100 calcium-binding protein β (S100β) staining showing astrocytes under distinct experimental conditions. m Density of Iba1+ microglial cells. n Density of S100β + astrocytes. o Density of Sox2- Vimentin- S100β + mature astrocytes. In a–l, Z-projection images are shown. ML Molecular layer. GCL Granule cell layer. H Hilus. Sox2 SRY (sex determining region Y)-box 2. White scale bar: 10 μm. Green triangles: Iba1+ microglial cells. Yellow triangles: S100β+ astrocytes. Graphs represent mean values ± SEM. n = 8 mice. +0.1 > p ≥ 0.05; *0.05 > p ≥ 0.01; and ***p < 0.001.