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. 2023 Sep 23;13:15889. doi: 10.1038/s41598-023-43221-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of differentially expressed genes in HCAECs cultured in the presence or absence of differentiated THP1 Mɸs and/or VSMCs. The differentially expressed genes identified by RNASeq in the comparison groups described in Fig. 1 were plotted as volcano plots to infer the overall distribution of differentially expressed genes in each treatment comparison group. In the figures, the x-axis shows the fold change in gene expression between different samples, and the y-axis shows the statistical significance of the differences. Significant gene expression changes (p ≤ 0.05; either up- or down-regulated) are shown in light grey. Non-significant protein changes are shown in black. The horizontal, dashed lines indicate the threshold line for differential gene screening criteria (p ≤ 0.05 and > twofold change).