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. 2023 Sep 23;13:15889. doi: 10.1038/s41598-023-43221-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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KEGG analysis of gene expression changes in HCAECs incubated with LPS-stimulated differentiated THP1 Mɸs, in the presence or absence of VSMCs. Upregulated gene expression from HCAECs, co-cultured with LPS-pre-treated differentiated THP1 Mɸs, in the absence (A) or presence (B) of VSMCs, were further analysed to identify common biological functions associated with gene expression changes by comparison with the Kyoto Encyclopaedia of Genes and Genomes (KEGG) database^54,55. From the KEGG enrichment results, the most significant 20 KEGG pathways were selected for display as a histogram, with the x-axis indicating the KEGG pathway, and the y-axis represents the significance level of pathway enrichment. The number of genes associated with each KEG pathway is indicated at the top of each bar.