FIG. 1.

Gel electrophoresis of amplification products with the nested 66-kDa protein gene primer set (PCR II) in skin samples. Lanes: 1, molecular standard, pUC HaeIII digest (587, 458, 434, 298, 267, 257, 174, 102, 80, 18, and 11); 2, positive control, 500 fg of ACA-1 DNA; 4, PET from patient no. 2 (EM); 6, FFT from patient no. 2; 8, sample from patient with other dermatological disease; 10, FFT from patient no. 37 (ACA); 12, therapy control; 14, PET from patient no. 12 (EM); 16, PET from patient no. 9 (EM). Negative controls are shown in unlabeled lanes 3, 5, 7, 9, 11, 13, and 15.