FIG. 3.

Gel electrophoresis of amplification products from urine specimens with the 23S rRNA primer set (PCR I). Lanes: 1, molecular standard, pUC HaeIII digest (587, 458, 434, 298, 267, 257, 174, 102, 80, 18, and 11); 2, positive control, 500 fg of ACA-1 DNA; 3, negative control; 4, specimen from patient no. 17 (EM); 5, specimen from patient no. 1 (EM); 6, specimen from patient no. 12 (EM); 7, specimen from patient no. 19 (ECM); 8, specimen from no. 13 (EM); 9, specimen from patient no. 15 (EM); 10, specimen from patient with other dermatological disease; 11, negative control; 12, specimen from patient no. 21 (ECM); 13, specimen from patient no. 3 (EM); 14, specimen from patient with dermatological disease other than LB; 15, specimen from patient no. 28 (ECM); 16, specimen from patient no. 38 (ACA).