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. 2023 Sep 23;14:5947. doi: 10.1038/s41467-023-41719-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Schematic representation of genetic elements in S. porcinus (sp) encoding ropB_sp, LCP_sp, and secreted cysteine protease speB_sp. The ropB_sp and LCP_sp are divergently transcribed. The bent arrow above indicates the transcription start site of LCP_sp. The numbers below denote the nucleotide positions relative to the first nucleotide of the start codon of speBsp. b The nucleotide sequence of the ropB_sp-LCP_sp intergenic region, coding sequence of LCP_sp, and corresponding predicted amino acid sequence of LCP_sp are shown. The ribosomal-binding sites (RBS) of LCP_sp and RopB_sp are marked by arrows. c Addition of synthetic LCP_sp causes early induction of speB expression in WT S. porcinus. speB transcript levels were assessed by qRT-PCR and the fold change in speB expression relative to unsupplemented growth (reference) is shown. d Schematics of genetic elements in E. malodoratus (em) encoding ropB_em, LCP_em, and genes encoding putative T7 secretion system (T7SS) and cognate effector (T7SS-eff). The bent arrow above indicates the predicted transcription start site (PLCP_em) of LCP_em. The numbers below denote the nucleotide positions relative to the first nucleotide of the start codon of LCP_em. e The nucleotide sequence of the ropB_em-LCP_em intergenic region, coding sequence of LCP_em, and corresponding predicted amino acid sequence of LCP_em are shown. The ribosomal-binding sites of LCP_em and RopB_em are underlined. f Addition of synthetic LCP_em causes early induction of T7SS and T7SS-eff expression in WT E. malodoratus. Transcript levels were assessed by qRT-PCR and the fold change in gene expression relative to unsupplemented growth (reference) is shown. In c, f, data are derived from three biological replicates analyzed in duplicate and data graphed represent mean values ± s.e.m. P values in (c, f) were calculated by Kruskal-Wallis test. In (c), * - P = 0.0189, n.s not significant. In panel f, ** - P = 0.0015, n.s - not significant, whereas in (g), ** - P = 0.0049, n.s - not significant. Source data are provided as a Source Data file.