Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 28;4(9):1326–1344. doi: 10.1038/s43018-023-00614-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a, Schematic of the intrasplenic model of liver colonization with early administration of treatment. CCs were implanted into the spleen of BALB/c-Fox1nuAusb mice under anesthesia on day 0. Treatment with 0.9% saline (vehicle); twice-weekly Gem (100 mg kg⁻¹ i.p.) (Gem); daily PXS-5505 at 20 mg kg⁻¹ i.p. (PXS-5505) or daily PXS-5505 at 20 mg kg⁻¹ i.p. + twice-weekly Gem at 100 mg kg⁻¹ i.p. (PXS-5505 + Gem) was administered from day 1. At the end point (day 10) livers were collected and H&E-stained for scoring. Representative images of H&E-stained liver (taken from one biologically independent animal). Scale bar, 100 μm. b, Representative images of H&E-stained livers from each treatment group (n = 1 FOV taken from one biologically independent animal per group). Scale bars, 100 μm. c, Quantification of metastases per mm² (n = 9 biologically independent animals per group). Data are mean ± s.d. Two-tailed P values were determined by unpaired, nonparametric t-test with a Mann–Whitney U-test correction (comparison between two groups). d, Quantification of the area of each metastasis in treatment groups (n = 9 biologically independent animals per group). Data are mean ± s.d. Two-tailed P values were determined by an unpaired, nonparametric t-test with a Mann–Whitney U-test correction (comparison between two groups). e, Schematic of intrasplenic model of liver colonization with late administration of treatment. CCs were implanted into the spleen of BALB/c-Fox1nuAusb mice under anesthesia on day 0. Treatment with 0.9% saline (vehicle); twice-weekly Gem (100 mg kg⁻¹ i.p.) (Gem); daily PXS-5505 at 20 mg kg⁻¹ i.p. (PXS-5505); or daily PXS-5505 at 20 mg kg⁻¹ i.p. + three doses of Gem (100 mg kg⁻¹ i.p. (PXS-5505 + Gem) was administered from day 4. Representative images of H&E-stained liver (taken from one biologically independent animal). Scale bar, 100 μm. f, Quantification of metastases at endpoint (n = 10 biologically independent animals per treatment group, n = 5 biologically independent animals at day 4 for confirmation of overt metastases). Data are mean ± s.d. P values were determined by an unpaired, nonparametric t-test with a Mann–Whitney U-test correction (comparison between two groups). g, Representative images of H&E-stained livers from each treatment group. Scale bars, 100 μm.

Source data