Figure 3.

CRP deficiency alters aortic leukocyte accumulation. (A): Representative aortic immunostaining images for CD68⁺ macrophages, CD4⁺ T cells, CD8⁺ T cells and B220⁺ B cells from non-aneurysmal normal aorta (left panels) and aneurysmal (wild type: middle panels; CRP^-/- mice: right panels). (B): Quantification of macrophage accumulation scores (media and interquartile) in WT and CRP^-/- aneurysmal aorta. N=10-11 mice per group, Non-parametric Mann-Whitney, *P<0.01 compared to WT mice. (C–E): Quantification of different lymphocyte subsets of aortic leukocytes [mean ± standard deviation for positive cells/aortic cross-section (ACS)] from the aneurysmal aortas of WT and CRP^-/- mice. No statistical difference in all lymphocyte subsets between two mouse strains.