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. 2023 Apr 30;9(2):82–98. doi: 10.52601/bpr.2023.230007

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The MS1 and MS2 setting for BoxCarmax-DIA. A The MS1 parameters and example of BoxCarmax MS1 (tSIM) scans. Among the four injections for one biological sample, each injection uses 60 min measurement time at a different set of boxcar MS1 windows (26 m/z isolation width). The representative total ion chromatograph (TIC) cross full MS1 scan and one MS1 spectrum are shown as examples. B The MS1 parameters and example of BoxCarmax MS2 scans. The first 10 scanning windows of 2.5 m/z each, as well as representative base peak chromatograph and one MS2 spectrum for one MSX are shown as examples of MS2 configuration. Please also see supplementary Table S1 for tables directly importable to Xcalibur