Figure 3.

Difference between plasma proteome profiles and EV proteome profiles

(A) Western blot of EVs isolated from plasma for conventional identified EV markers.

(B) Box plot showing the proteins identified in normal and UTUC EV samples. Boxplots show median (central line), upper and lower quartiles (box limits), 1.5× interquartile range (whiskers).

(C) Venn diagram showing proteins identified in EVs herein and belonging to human EV proteins in the Vesiclepedia dataset.

(D) Venn diagram showing proteins identified in more than 30% of UTUC plasma observed in EVs.

(E) Venn diagram showing proteins upregulated in UTUC plasma (left) or downregulated in UTUC plasma (right) observed in EVs.

(F) Volcano plot showing the EV protein abundance differences between UTUC and normal plasma samples.

(G) Bubble plot showing pathways enriched in normal and UTUC samples.

(H) Venn diagram of upregulated or downregulated proteins identified in both EVs and plasma.

(I) Heatmap of EV proteins abundance differences between normal and UTUC samples.

(J) Box plot showing EV protein abundance of PSMD2 (BH P = 0.028) and ICAM1 (BH P = 0.040).